Phospholipidosis assay
WebJun 21, 2024 · 1. Introduction. Drug-induced phospholipidosis (DIPLD) is characterized by excessive intracellular accumulation of phospholipids as a result of drug treatments [1].Since the first DIPLD case reported in 1948 [2], an increasing number of drugs were identified as PLD inducers, including antibiotics, antidepressants, antipsychotics and so … WebFluorescent images were captured and analyzed using the Discovery-1 automated cellular imaging system. Eleven out of the 12 selected positive compounds and all negative compounds were properly assigned as positive and negative inducers of phospholipidosis, respectively, indicating the high degree of sensitivity and specificity of this assay.
Phospholipidosis assay
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Webcontent phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) plat- form. This assay has been optimized and validated in HepG2 and HepRG cells, and miniaturized into a WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high ...
WebPhospholipidosis (PL) is lysosomal storage disorder characterized by the excessive accumulation of intracellular phospholipids (Figure 1). The mechanism of phospholipidosis is still unclear today, but it is known that phospholipidosis is commonly caused by the intake of a special group of drugs containing a hydrophobic ring/chain and a hydrophilic amine … WebFeb 1, 2007 · Phospholipidosis was determined by algorithms that evaluated the granular texture of the perinuclear area as an indicator of the amount of phospholipidosis vacuoles. ... High-content screening...
WebSep 3, 2013 · In this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. … WebCerep Phospholipidosis assay (HepG2 cells) PubChem. 2 Comment. Help. New Window. Compounds with activity <= 10uM or explicitly reported as active by ChEMBL are flagged as active in this PubChem assay presentation. Target ChEMBL ID: CHEMBL1626541. ChEMBL Target Name: Phospholipidosis.
WebNational Center for Biotechnology Information great downtownsWebinstaCELL® Phospholipidosis Assay Kits. Drug induced accumulation of phospholipids (phospholipidosis) is associated with clinical toxicities. Cell‐based assays can help to … great doxologyWebDec 1, 2011 · Phospholipidosis is a lipid storage disorder which is recognized by excessive accumulation of phospholipids in the lysosomes within cells. Phospholipidosis can result from hereditary predisposition, which is for example the case in Niemann-Pick disease, but may also be the result of drug exposure. great dragon buffet phoenixWebJul 7, 2004 · Required concentrations for increased Nile Red binding were generally three-fold or more lower than the cytotoxic concentration determined by a resazurin reduction assay in the same cells. Qualitatively similar Nile Red binding results were obtained when primary canine or rat hepatocytes were used. great dragon rheumatism oilWebIn this study, a cell-based phospholipidosis assay has been developed using the LipidTOX Red reagent in HepG2 cells and miniaturized into a 1536-well plate format. To validate this assay for high-throughput screening (HTS), the LOPAC library of 1280 compounds was screened using a quantitative HTS platform. A group of known phospholipidosis ... great dragon\\u0027s head p99WebAug 2010 - Oct 20144 years 3 months. Jalandhar,Punjab,India. The active area of research at University was Metabolic disorder (Diabetes).I was involved in in-vitro selection & characterization, target validation for metabolic targets, assay development and condition optimization, its high throughput screening, selection of hits among phyto ... great dragon decorah iowaWebMar 17, 2024 · This chapter describes a cell-based high-content phospholipidosis assay using the LipidTOX reagent in a high-throughput screening (HTS) platform. This assay has … great dragon somber smithing stone